Young Coconut Juice Reduces Some Histopathological Changes Associated with Alzheimer’s Disease through the Modulation of Estrogen Receptors in Orchidectomized Rat Brains

Table 1

Antibodies, concentration, and manufacturer used for brain sections of each rat.

Section No.	Staining
1-2	Cresyl violet, for histological orientation
3-4	Anti-NF200 antibody, 1 : 2,000 diluted (N0142, Sigma-Aldrich, USA)
5-6	Anti-PV antibody, 1 : 1,000 diluted (P3088, Sigma-Aldrich, USA)
7-8	Anti-β-amyloid 1-42 polyclonal antibody, 1 : 50 diluted (AB5078P, Chemicon, USA)
9-10	Mouse anti-estrogen receptor α (aa-120-170) antibody, 1 : 500 diluted (MAB447, Chemicon international, USA)
11-12	Estrogen receptor β antibody, 1 : 2,000 diluted (PA1-310B, Thermo Fisher Scientific, USA)
13-14	Immunostaining, omitting primary antibodies (negative control)