Insulin-Like Growth Factor I Prevents Cellular Aging via Activation of Mitophagy

Figure 1

IGF-1 reversed aging phenotype of long-term cultured vascular SMC. Aortic SMCs were isolated from control mice (Con SMC) and mice with IGF-1 receptor deficiency (IGF-1R-KO SMC) and cultured until passage 5 (P5) or passage 20 (P20). Cells were exposed to IGF-1 for 12 h, and activity of senescence-associated β-galactosidase (SA-β-Gal) (a), cytochrome C oxidase (b), mitochondrial membrane potential (MMP) (c), mitochondrial DNA damage (d), and DNA telomere length (e) was quantified using commercial assays in accordance with manufacturer’s instructions. for P20 vs. P5 SMC. ^# for IGF-1-treated P20 SMC vs. untreated P20 SMC. Empty bars, P5 SMC; solid bars, P20 SMC.

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