Insulin-Like Growth Factor I Prevents Cellular Aging via Activation of Mitophagy
IGF-1 upregulated autophagy in P20 SMC. P20 SMCs were treated with 10 ng/ml IGF-1, with 10 μM Bafilomycin (Baf), or with combination of IGF-1 and Baf and stained with dye labelling autophagic vacuoles and counterstained with DAPI (a, b). Autophagic dye signal was localized in spherical vacuoles in the perinuclear region of the cell and in foci distributed throughout the cytoplasm. Data were shown as a total number of autophagic vacuoles per cell. LC3 II/I ratio was quantified by immunoblotting/densitometry (c, d), and the results were normalized per β-actin levels. for IGF-1-treated vs. untreated cells. Scale bar, 100 μm.
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