Age- and Organ-Specific Differences in Mitochondrial Bioenergetics in Brown Norway Rats

Figure 5

Typical traces of brain (frontal cortex) mitochondrial bioenergetics using Seahorse Extracellular Flux Analyzer from rats that are 1 month, 4 months, 12 months, and 24 months of age. Each line shown is representative point-to-point display of patterns of OCR respiration rates at 1, 4, 12, or 24 months of age. Oxygen consumption rates (OCR) were measured with addition of substrates/inhibitors of electron transport chain. Vertical lines represent addition of compounds used to determine the various respiration states: (A) substrates (pyruvate, malate, and adenosine diphosphate) for State III; (B) oligomycin for State IV; (C) carbonyl cyanide 4-(trifluoromethoxy)phenylhydrazone (FCCP) for State V₁; and (D) succinate and rotenone for State V₂.