UVA Exposure Combined with Glycation of the Dermis Are Two Catalysts for Skin Aging and Promotes a Favorable Environment to the Appearance of Elastosis

Figure 4

Specific effect of pentosidine on fibroblast monolayers. Determination of IL8 (a), CXCL2 (b), and MMP12 (c) gene expression in fibroblast monolayers (2D culture) from young or old donors after stimulation by pentosidine (young (T) or old (T) during 48 h). mRNA levels were quantified in fibroblasts using qPCR. Each point represents the mean value of normalized mRNA quantity (n = 3). qPCR was performed in duplicate on three different samples. Data are expressed in arbitrary units (AU ± SD = arbitrary units ± standard deviation). Tropoelastin immunostaining (d) was observed in fibroblasts monolayers after pentosidine stimulation at 0, 1, and 10 μM. Note that pentosidine stimulates tropoelastin expression by fibroblasts. Statistics: analysis of variance (ANOVA) adjusted post hoc Tukey–Kramer tests for pairwise comparisons ( < 0.05; < 0.0001).

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