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Journal of Botany
Volume 2017, Article ID 4369029, 16 pages
https://doi.org/10.1155/2017/4369029
Research Article

Phylogeny of Ten Kenyan Plectranthus Species in the Coleus Clade Inferred from Leaf Micromorphology, Rbcl and MatK Genes

1Department of Applied and Technical Biology, School of Biological and Life Sciences, Technical University of Kenya, P.O. Box 52428-00200, Nairobi, Kenya
2School of Biological Sciences, College of Biological and Physical Sciences, University of Nairobi, P.O. Box 30197-00100, Nairobi, Kenya
3Department of Public Health, Toxicology and Pharmacology, College of Agriculture and Veterinary Sciences, University of Nairobi, P.O. Box 29053-00625, Nairobi, Kenya

Correspondence should be addressed to Fredrick M. Musila; moc.liamg@eitumalisum

Received 22 July 2017; Revised 31 October 2017; Accepted 20 November 2017; Published 18 December 2017

Academic Editor: Jutta Ludwig-Müller

Copyright © 2017 Fredrick M. Musila et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Linked References

  1. L. J. Rice, G. J. Brits, C. J. Potgieter, and J. Van Staden, “Plectranthus: a plant for the future?” South African Journal of Botany, vol. 77, no. 4, pp. 947–959, 2011. View at Publisher · View at Google Scholar · View at Scopus
  2. C. W. Lukhoba, M. S. J. Simmonds, and A. J. Paton, “Plectranthus: a review of ethnobotanical uses,” Journal of Ethnopharmacology, vol. 103, no. 1, pp. 1–24, 2006. View at Publisher · View at Google Scholar · View at Scopus
  3. R. J. Grayer, M. R. Eckert, A. Lever, N. C. Veitch, G. C. Kite, and A. J. Paton, “Distribution of exudate flavonoids in the genus Plectranthus,” Biochemical Systematics and Ecology, vol. 38, no. 3, pp. 335–341, 2010. View at Publisher · View at Google Scholar · View at Scopus
  4. A. J. Paton, D. Springate, S. Suddee et al., “Phylogeny and evolution of basils and allies (Ocimeae, Labiatae) based on three plastid DNA regions,” Molecular Phylogenetics and Evolution, vol. 31, no. 1, pp. 277–299, 2004. View at Publisher · View at Google Scholar · View at Scopus
  5. A. J. Paton, G. Bramley, O. Rying et al., Flora of Tropical East Africa Lamiaceae (Labaiate), Royal Botanical Gardens, Kew, 2009.
  6. F. Al-Qurainy, S. Khan, M. Nadeem, M. Tarroum, and A. Al-Ameri, “Selection of DNA barcoding loci and phylogenetic study of a medicinal and endemic plant, Plectranthus asirensis J.R.I. wood from Saudi Arabia,” Genetics and Molecular Research, vol. 13, no. 3, pp. 6184–6190, 2014. View at Publisher · View at Google Scholar · View at Scopus
  7. J. D. M. Bandeira, V. J. Bianchi, S. Rubin, J. A. Peters, and E. J. B. Braga, “Genetic similarities among four species of the Plectranthus (L’Hér.) genus,” Acta Scientiarum - Biological Sciences, vol. 32, no. 1, pp. 43–48, 2010. View at Publisher · View at Google Scholar · View at Scopus
  8. A. P. P. R. Amarasinghe, D. A. S. Siriwardhane, R. R. Samarasekera, and O. V. D. S. J. Weerasena, “Development of DNA barcodes and optimization of DNA isolation and PCR Protocols for a medicinal plant Plectranthus hadiensis,” International Journal of Science and Research, vol. 4, no. 6, pp. 2852–2856, 2013. View at Google Scholar
  9. H.-K. Moon, S.-P. Hong, E. Smets, and S. Huysmans, “Phylogenetic significance of leaf micromorphology and anatomy in the tribe Mentheae (Nepetoideae: Lamiaceae),” Botanical Journal of the Linnean Society, vol. 160, no. 2, pp. 211–231, 2009. View at Publisher · View at Google Scholar · View at Scopus
  10. A. Bisio, A. Corallo, P. Gastaldo et al., “Glandular hairs and secreted material in Salvia blepharophylla Brandegee ex Epling grown in Italy,” Annals of Botany, vol. 83, no. 4, pp. 441–452, 1999. View at Publisher · View at Google Scholar · View at Scopus
  11. G. Corsi and S. Bottega, “Glandular hairs of Salvia officinalis: new data on morphology, localization and histochemistry in relation to function,” Annals of Botany, vol. 84, no. 5, pp. 657–664, 1999. View at Publisher · View at Google Scholar · View at Scopus
  12. R. F. Evert, Esau’s Plant Anatomy: Meristems, Cells, and Tissues of The Plant Body: Their Structure, Function, and Development, John Wiley & Sons, 2006.
  13. A. Cronquist, An Integrated System of Classification of Flowering Plants, Columbia University Press, 1981.
  14. M. F. Duarte and J. F. Lopes, “Stem and leaf anatomy of Plectranthus neochilus Schltr., Lamiaceae,” Revista Brasileira de Farmacognosia, vol. 17, no. 4, pp. 549–556, 2007. View at Publisher · View at Google Scholar · View at Scopus
  15. C. R. Metcalfe and L. Chalk, Anatomy of The Dicotyledons, Oxford: Clarendon Press, Oxford, UK, 2nd edition, 1988.
  16. F. E. Fritsch, Anatomy of the Dicotyledons. Leaves, Stem and Wood in Relation to Taxonomy, with Notes on Economic Uses. Vols. I and II, Oxford at the Clarendon Press, 1950.
  17. E. Werker, “Function of essential oil‐secreting glandular hairs in aromatic plans of Lamiacea—a review,” Flavour and Fragrance Journal, vol. 8, no. 5, pp. 249–255, 1993. View at Publisher · View at Google Scholar · View at Scopus
  18. L. Ascensão, L. Mota, and M. D. M. Castro, “Glandular trichomes on the leaves and flowers of Plectranthus ornatus: morphology, distribution and histochemistry,” Annals of Botany, vol. 84, no. 4, pp. 437–447, 1999. View at Publisher · View at Google Scholar · View at Scopus
  19. K. N. A. Khalik and E. A. Karakish, “Comparative anatomy of stems and leaves of plectranthus L. (Lamiaceae) in Saudi Arabia and systematic implications,” Microscopy Research and Technique, vol. 79, no. 7, pp. 583–594, 2016. View at Publisher · View at Google Scholar · View at Scopus
  20. P. Catalán, Y. Shi, L. Armstrong, J. Draper, and C. A. Stace, “Molecular phylogeny of the grass genus Brachypodium P. Beauv. based on RFLP and RAPD analysis,” Botanical Journal of the Linnean Society, vol. 117, no. 4, pp. 263–280, 1995. View at Publisher · View at Google Scholar · View at Scopus
  21. M. Abdulla and O. Gamal, “Investigation on molecular phylogeny of some date palm (Phoenix dactylifra L.) cultivars by protein, RAPD and ISSR markers in Saudi Arabia,” Australian Journal of Crop Science, vol. 4, no. 1, pp. 23–28, 2010. View at Google Scholar · View at Scopus
  22. M. Hajibabaei, G. A. C. Singer, P. D. N. Hebert, and D. A. Hickey, “DNA barcoding: how it complements taxonomy, molecular phylogenetics and population genetics,” Trends in Genetics, vol. 23, no. 4, pp. 167–172, 2007. View at Publisher · View at Google Scholar · View at Scopus
  23. F. De Mattia, I. Bruni, A. Galimberti, F. Cattaneo, M. Casiraghi, and M. Labra, “A comparative study of different DNA barcoding markers for the identification of some members of Lamiacaea,” Food Research International, vol. 44, no. 3, pp. 693–702, 2011. View at Publisher · View at Google Scholar · View at Scopus
  24. I. Bruni, A. Galimberti, L. Caridi et al., “A DNA barcoding approach to identify plant species in multiflower honey,” Food Chemistry, vol. 170, pp. 308–315, 2015. View at Publisher · View at Google Scholar · View at Scopus
  25. M. Li, H. Cao, P. P. H. But, and P. C. Shaw, “Identification of herbal medicinal materials using DNA barcodes,” Journal of Systematics and Evolution, vol. 49, no. 3, pp. 271–283, 2011. View at Publisher · View at Google Scholar · View at Scopus
  26. K. Tamura, G. Stecher, D. Peterson, A. Filipski, and S. Kumar, “MEGA6: Molecular Evolutionary Genetics Analysis version 6.0,” Molecular Biology and Evolution, vol. 30, no. 12, pp. 2725–2729, 2013. View at Publisher · View at Google Scholar
  27. R. C. Edgar, “MUSCLE: multiple sequence alignment with high accuracy and high throughput,” Nucleic Acids Research, vol. 32, no. 5, pp. 1792–1797, 2004. View at Publisher · View at Google Scholar · View at Scopus
  28. P. H. A. Sneath and R. R. Sokal, Numerical Taxonomy: The Principles and Practice of Numerical Classification, W. H. Freeman, San Francisco, Calif, USA, 1973. View at MathSciNet
  29. B. Efron and R. J. Tibshirani, An Introduction to the Bootstrap, Monographs on Statistics and Applied Probability, Chapman and Hall, New York, NY, USA, 1993. View at Publisher · View at Google Scholar · View at MathSciNet
  30. A. P. Davis and J. R. Barnett, “The leaf anatomy of the genus Galanthus L. (Amaryllidaceae J. St.-Hill),” Botanical Journal of the Linnean Society, vol. 123, no. 4, pp. 333–352, 1997. View at Publisher · View at Google Scholar · View at Scopus
  31. M. G. Al-Saghir, D. M. Porter, and E. T. Nilsen, “Leaf anatomy of Pistacia species (Anacardiaceae),” Journal of Biological Sciences, vol. 6, no. 2, pp. 242–244, 2006. View at Publisher · View at Google Scholar · View at Scopus
  32. U. K. Abdel-Hameed, M. E. Tantawy, M. A. Salim, M. M. Mourad, and I. F. Ishak, “Phenetic Analysis of Morphological and Molecular Traits in Acanthaceae Juss,” Journal of Biosciences and Medicines, vol. 3, no. 3, pp. 18–34, 2015. View at Publisher · View at Google Scholar
  33. O. Yim and K. T. Ramdeen, “Hierarchical cluster analysis: comparison of three linkage measures and application to psychological data,” The Quantitative Methods for Psychology, vol. 11, no. 1, pp. 8–21, 2015. View at Publisher · View at Google Scholar
  34. L. D. Gottlieb, “Electrophoretic evidence and plant populations,” Progress in Phytochemistry, vol. 7, no. 1, p. 46, 1981. View at Google Scholar
  35. K. N. Abdel Khalik, “A systematic revision of the genus Plectranthus L. (Lamiaceae) in Saudi Arabia based on morphological, palynological, and micromorphological characters,” American Journal of Plant Sciences, vol. 7, no. 10, p. 1429, 2010. View at Google Scholar
  36. N. D. Kaliappan and P. K. Viswanathan, “Pharmacognostical studies on the leaves of Plectranthus amboinicus (Lour) Spreng,” International Journal of Green Pharmacy, vol. 2, no. 3, pp. 182–184, 2008. View at Google Scholar
  37. C. P. Klingenberg, “Evolution and development of shape: integrating quantitative approaches,” Nature Reviews Genetics, vol. 11, no. 9, pp. 623–635, 2010. View at Publisher · View at Google Scholar · View at Scopus
  38. Y. Salmaki, S. Zarre, Z. Jamzad, and C. Bräuchler, “Trichome micromorphology of Iranian Stachys (Lamiaceae) with emphasis on its systematic implication,” Flora, vol. 204, no. 5, pp. 371–381, 2009. View at Publisher · View at Google Scholar · View at Scopus
  39. C.-L. Xiang, Z.-H. Dong, H. Peng, and Z.-W. Liu, “Trichome micromorphology of the East Asiatic genus Chelonopsis (Lamiaceae) and its systematic implications,” Flora, vol. 205, no. 7, pp. 434–441, 2010. View at Publisher · View at Google Scholar · View at Scopus
  40. E. O. Wiley, Phylogenetics. The Principles and Practice of Phylogenetic Systematics, Wiley J & Sons, New York, NY, USA, 1981.
  41. D. M. Hillis and J. J. Wiens, “Molecules versus morphology in systematics: conflicts, artifacts, and misconceptions,” in Phylogenetic Analysis of Morphological Data, pp. 1–19, 2000. View at Google Scholar
  42. M. K. Krause, “Molecular approaches and the growth of phylogenetic biology,” in Molecular Zoology: Advances, Strategies, and Protocols, J. D. Ferraris and S. R. Palumbi, Eds., pp. 47–63, Wiley-Liss, New York, NY, USA, 1996. View at Google Scholar
  43. F. M. Musila, C. W. Lukhoba, S. F. Dossaji, and J. M. Nguta, “Molecular Phylogeny of ten Kenyan Plectranthus species in the Coleus clade,” in Proceedings of the XXI AETFAT congress, Catholic University of Eastern Africa, Nairobi, Kenya, 2017.