The Investigation of Effects of Quercetin and Its Combination with Cisplatin on Malignant Mesothelioma Cells In Vitro

Figure 3

SPC212 cells ((a) and (b)) and SPC111 cells ((c) and (d)) were untreated, treated with 5 𝜇g/mL CIS, 10 𝜇g/mL CIS, 50 𝜇M QU, and combination of both at 24 hours ((a) and (c)) and 48 hours ((b) and (d)). Accumulation of cells in S phase was detected in SPC212 but not in SPC111 cells following QU treatments for 48 hours ((b) and (d)). However, individual CIS applications resulted in accumulation of both cells in S phase at the first 24 hours period ((a) and (c)), and cells were arrested at G₂/M phase in the following 24 hours ((b) and (d)). Distribution of cell cycle was altered in both cells with 5 𝜇g/mL CIS + 50 𝜇M QU treatment compared with untreated cells and cells were treated with individual agents. The percentage of S phase arrest was observed as 86.7% in SPC212 and 99% in SPC111 cells, at 48 hours ((b) and (d)). On the other hand, cells treated with 10 𝜇g/mL CIS + 50 𝜇M QU showed a similar profile to that of individual 10 𝜇g/mL CIS dose applications in SPC111 cells ((c) and (d)).

(a)

(b)

(c)

(d)