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E-Journal of Chemistry
Volume 4 (2007), Issue 4, Pages 546-549

Isolation and Identification of Anthralin From the Roots of Rhubarb Plant (Rheum palmatum)

A. Ashnagar,1 N. Gharib Naseri,2 and H. Haidari Nasab1

1School of Pharmacy, Ahwaz Jundi Shapour Univ. of Medical Sciences, Ahwaz, Iran
2Ahwaz Faculty of Petroleum Engineering, Petroleum Univ. of Technology, Iran

Received 4 March 2007; Accepted 30 March 2007

Copyright © 2007 Hindawi Publishing Corporation. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Anthralin is medically and chemically an important compound which can be found in rhubarb roots. Anthralin and anthralin derivatives have been used as antipsoriatic drugs. In this research, pure anthralin was isolated from the rhubarb roots by maceration and Soxhlet extraction methods in various polar and non-polar solvents. Successive TLC and column chromatography on silica gel with chloroform as the mobile phase afforded two distinct fractions with Rf= 0.54 and 0.61. The 1HNMR, 13CNMR, IR, UV and MS spectra showed that the fraction with Rf= 0.61 was anthralin. In both methods, methanol was found to be the most suitable solvent for extraction.