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E-Journal of Chemistry
Volume 5, Issue 4, Pages 770-773

Estimation of Chlorpyriphos in its Formulation (Paraban 20% EC) by Reversed-Phase HPLC

Deepti Joshi1 and N. K. Sand2

1Department of Chemistry, M.B.P.G.College, Kumaun University, Haldwani, Uttaranchal, India
2Department of Chemistry, College of Basic Sciences and Humanities, G.B.Pant University of Agriculture & Tecnology, Pantnagar, U.S.Nagar, Uttaranchal, India

Received 3 January 2008; Accepted 1 March 2008

Copyright © 2008 Hindawi Publishing Corporation. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


A method has been developed for estimation of active ingredient in chlorpyriphos formulation (Paraban 20% EC). The formulation was extracted in chloroform, dried and resuspended in acetonitrile. The cleanup was performed using C18 SPE cartridge. The active ingredient was estimated using 5 μm ODS-II column, mobile phase was acetonitrile: water (75:25 v/v) and detection at 229nm. The efficiency of clean up method was found to be 95% and minimum limit of detection < 2.5ng. The detector response was linear with in concentration range 2.5ng – 50ng at RSD 1.42%.