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E-Journal of Chemistry
Volume 5 (2008), S2, Pages 1081-1086

A Simple and Rapid RP-HPLC Method for the Estimation of Nevirapine in Bulk and Pharmaceutical Dosage Forms

Palaniappan Mohanraj, Deb Kumar Sarkar, Tirthankar Choudhury, and Karunakaran Gauthaman

Department of Pharmaceutical Sciences, Himalayan Pharmacy Institute, E.Sikkim-737136, India

Received 9 March 2008; Accepted 22 April 2008

Copyright © 2008 Hindawi Publishing Corporation. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


A reverse phase HPLC method is described for the determination of nevirapine in tablet dosage forms. Chromatography was carried on an ODS column using a mixture of methanol and water (89:11 v/v) as the mobile phase at a flow rate of 1 mL/min with detection at 284 nm. The retention time of the drug was 3.22 min. The detector response was linear in the concentration of 25-200 mcg/mL. The limit of detection and limit of quantification was 0.004 and 0.0121 mcg/mL respectively. The percentage assay of nevirapine was 99.52%. The method was validated by determining its sensitivity, accuracy and precision. The proposed method is simple, economical, fast, accurate and precise and hence can be applied for routine quality control of nevirapine in bulk and tablet dosage forms.