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E-Journal of Chemistry
Volume 5, Issue 1, Pages 68-73
http://dx.doi.org/10.1155/2008/637235

Isolation and Characterization of Process-Related Impurity in Azoxystrobin

S. G. Hiriyanna,1 K. Basavaiah,1,2 and K. Sreedhar2

1Department of Chemistry, Manasagangothri, University of Mysore, Mysore-570009, India
2Department of Chemistry, Kakathiya University, Warrangal, India

Received 26 April 2007; Accepted 31 May 2007

Copyright © 2008 Hindawi Publishing Corporation. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

One unknown impurity in azoxystrobin bulk material at a level 0.15% was detected by a gradient reverse phase high performance liquid chromatography (HPLC). This impurity was isolated from a crude sample of azoxystrobin using reverse phase preparative LC. The impurity isolated by preparative LC was characterized by NMR, MS experimental data. Based on the results obtained from different spectroscopic experiments, these impurity was characterized as methyl 2-(2- (6- (2-cyanophenoxy)-2-((4-(2-cyanophenoxy)-6- (2- (1, 3-dimethoxy-3-oxoprop-l-en-2-yl)phenoxy) pyrimidin-5-yl) methyl)pyrimidin-4-yloxy)phenyl)-3-methoxyacrylate. Formation of this impurity is also discussed.