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E-Journal of Chemistry
Volume 6 (2009), Issue 4, Pages 1071-1076
http://dx.doi.org/10.1155/2009/304925

A Novel Method to Prepare PolyGMA and its Application to Immobilization of β-Galactosidase

Sun Sufang and Dong Lingyun

College of Chemistry and Environmental Science, Hebei University, Baoding 071002, China

Received 17 January 2009; Accepted 6 March 2009

Copyright © 2009 Hindawi Publishing Corporation. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

The reactive, macroporous and massive epoxy group containing polyGMA was synthesized simultaneously with methanol aqueous solution and nano-calcium carbonate as porogen by bulk copolymerization. After the polymer was smashed, particles with diameters in the range of 0.30 to 0.45 mm were taken as the carrier and the scanning electron microscopy (SEM) micrographs were done to characterize its surface structure. Under the optimum conditions, β-galactosidase was immobilized on the supporter obtained above and the enzyme activity bound per gram drier carrier could attained up to 77.33%, which was much higher than that bound on the carrier prepared by only using methanol aqueous solution as porogen. The conclusion obtained indicated that the ployGMA prepared concurrently with liquid and solid porogen was more suitable to immobilize enzyme because of the much more porous surface structure obtained.