G. Vijaya Raja, C. Bala Sekaran, D. Winnie Teja, B. Madhuri, B. Jayasree, "Simple Spectrophotometric Methods for the Determination of Sulfamethaxazole in Pharmaceuticals Using Folinciocalteau and Orcinol as Reagents", Journal of Chemistry, vol. 6, Article ID 457130, 4 pages, 2009. https://doi.org/10.1155/2009/457130
Simple Spectrophotometric Methods for the Determination of Sulfamethaxazole in Pharmaceuticals Using Folinciocalteau and Orcinol as Reagents
Two simple, sensitive and reproducible spectrophotometric methods (Method A and Method B) were developed for the determination of sulfamethaxazole in bulk and in dosage forms. Method A is based reduction of phosphomolybdic acid present in Folin Ciocalteau reagent by the drug sulfamethaxazole in the presence of sodium carbonate to form a blue colored chromogen having maximum absorption at 760 nm. Method B is based on the diazotization of the drug by sodium nitrite in acidic medium at 5 °C followed by coupling with orcinol to form yellow colored species (λmax 390 nm). Beer’s law is obeyed in the range of 5-25 μg/mL for method A and 2-10 μg/mL for method B. Results of analysis were validated statistically and by recovery studies. These methods are successfully employed for the determination of sulfamethaxazole in various pharmaceutical preparations and biological fluids.
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