Journal of Chemistry

Journal of Chemistry / 2009 / Article

Open Access

Volume 6 |Article ID 457130 | 4 pages |

Simple Spectrophotometric Methods for the Determination of Sulfamethaxazole in Pharmaceuticals Using Folinciocalteau and Orcinol as Reagents

Received03 Sep 2008
Accepted05 Oct 2008


Two simple, sensitive and reproducible spectrophotometric methods (Method A and Method B) were developed for the determination of sulfamethaxazole in bulk and in dosage forms. Method A is based reduction of phosphomolybdic acid present in Folin Ciocalteau reagent by the drug sulfamethaxazole in the presence of sodium carbonate to form a blue colored chromogen having maximum absorption at 760 nm. Method B is based on the diazotization of the drug by sodium nitrite in acidic medium at 5 °C followed by coupling with orcinol to form yellow colored species (λmax 390 nm). Beer’s law is obeyed in the range of 5-25 μg/mL for method A and 2-10 μg/mL for method B. Results of analysis were validated statistically and by recovery studies. These methods are successfully employed for the determination of sulfamethaxazole in various pharmaceutical preparations and biological fluids.

Copyright © 2009 Hindawi Publishing Corporation. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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