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E-Journal of Chemistry
Volume 6, Issue 4, Pages 979-984

Effect of Fermentation Parameters on Extra Cellular Tannase Production by Lactobacillus plantarum MTCC 1407

Kannan Natarajan1 and Aravindan Rajendran2

1Department of Biotechnology, Manipal Institute of technology, Manipal-576104, Karnataka, India
2Biochemical Engineering Laboratory, Department of Chemical Engineering, Annamalai University, Annamalai Nagar-608 002, Tamilnadu, India

Received 12 December 2008; Accepted 25 February 2009

Copyright © 2009 Hindawi Publishing Corporation. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Lactobacillus plantarum MTCC 1407 represents a valuable source of an economically attractive stable long-life tannase with potential for application in various industries. The effect of fermentation parameters such as pH, temperature and agitation speed on the growth of biomass and production of tannase using liquid medium were determined at the end of fermentation period. The optimum values of pH, reaction temperature and agitation speed for tannase activity were 6.0, 30 °C and 125 rpm respectively. The maximum tannase activity was found to be 9.29 U/mL.