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E-Journal of Chemistry
Volume 7 (2010), Issue 4, Pages 1539-1545
http://dx.doi.org/10.1155/2010/137178

Spectrophotometric Methods for the Assay of Fluvoxamine Using Chromogenic Reagents

V. Annapurna,1 G. Jyothi,1 V. Nagalakshmi,1 and B. B. V. Sailaja2

1Department of Chemistry, St. Theresa’s College for Women, Eluru-534003, India
2Andhra University, Visakhapatnam, Andhra Pradesh, India

Received 25 November 2009; Accepted 20 January 2010

Copyright © 2010 Hindawi Publishing Corporation. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Simple, accurate and reproducible UV-Visible spectrophotometric methods were established for the assay of FXA based on the oxidative coupling and condensation reactions. Condensation and coupling of the FXA with Ninhydrin-Ascorbic acid is proposed in method A. Method B includes complexation of FXA with cobalt thiocyanate. The ligating property of FXA with sodium nitro prusside is incorporated in method C. The optical characteristics such as Beers law limits, molar absorptivity and Sandell’s sensitivity for the methods (A-C) are given. Regression analysis using the method of least squares was made to evaluate the slope(b), intercept(a) and correlation coefficient (r) and standard error of estimation (Se) for each system. Determination of FXA in bulk form and in pharmaceutical formulations were also incorporated.