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E-Journal of Chemistry
Volume 7, Issue 3, Pages 915-921

Extractive Spectrophotometric Method for Determination of Pioglitazone Hydrochloride in Raw Material and Tablets Using Ion-Pair Formation

Massoud Amanlou,1 Mohadeseh Zarei-Ghobadi,2 Mohammad Kazem Rofouei,2 Shahrooz Saremi,3 and Abbas Kebriaeezadeh4

1Drug Design & Development Research Center, Tehran University of Medical Sciences, Tehran, Iran
2Department of Chemistry, Tarbiat Moallem University, Tehran, Iran
3Department of Pharmaceutics, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran
4Departments of Pharmacology & Toxicology, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran

Received 31 October 2009; Accepted 20 December 2009

Copyright © 2010 Hindawi Publishing Corporation. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


A simple, rapid and extractive spectrophotometric method was developed for the determination of pioglitazone hydrochloride in pure and pharmaceutical formulations. This method is based on the formation of yellow ion-pair complex between the basic nitrogen of the drug and bromocresol green (BCG) in phthalate buffer of pH 2.4. The formed complexes were extracted with chloroform and measured at 419 nm. The analytical parameters and their effects on the proposed systems are investigated. Beer’s law was obeyed in the range 2.5-14 μg/mL with correlation coefficient ≥ 0.995. The proposed method has been applied successfully for the determination of drug in commercial tablets dosage forms. No significant interference was observed from the excipients commonly used as pharmaceutical aids with the assay procedure. The validity of the proposed method was established by parallel determination against HPLC method and there was no significant difference between these two methods.