Journal of Chemistry

Journal of Chemistry / 2010 / Article

Open Access

Volume 7 |Article ID 490328 | https://doi.org/10.1155/2010/490328

S. K. Patro, S. R. Swain, V. J. Patro, N. S. K. Choudhury, "Development and Validation of High Performance Liquid Chromatographic Method for Determination of Lamivudine from Pharmaceutical Preparation", Journal of Chemistry, vol. 7, Article ID 490328, 6 pages, 2010. https://doi.org/10.1155/2010/490328

Development and Validation of High Performance Liquid Chromatographic Method for Determination of Lamivudine from Pharmaceutical Preparation

Received22 Jun 2009
Accepted15 Aug 2009

Abstract

A new, simple, specific, accurate and precise RP-HPLC method was developed for determination of lamivudine in pure and tablet formulations. A Thermo BDS C18 column in isocratic mode, with a mobile phase consisting of 0.01 M ammonium dihydrogen orthophosphate buffer adjusted to pH 2.48 by using formic acid and methanol in the ratio of 50:50 was used. The flow rate was set at 0.6 mL/min and UV detection was carried out at 264 nm. The retention time of lamivudine and nevirapine were 2.825 min and 4.958 min respectively. The method was validated for linearity, precision, robustness and recovery. Linearity for lamivudine was found in the range of 50-175 μg/mL. Hence, it can be applied for routine quality control of lamivudine in bulk and pharmaceutical formulations.

Copyright © 2010 Hindawi Publishing Corporation. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


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