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E-Journal of Chemistry
Volume 7, Issue 3, Pages 962-966

Estimation of Atenolol by Reverse Phase High Performance Liquid Chromatography

Naveen Kumar,1 Nishant Verma,1 Omveer Songh,1 Naveen Joshi,1 and Kanwar Gaurav Singh2

1School of Pharmaceutical Sciences, Shobhit University, Meerut-250110, India
2Singhania University Pacheri Bari, Distt. Jhunjhunu (Rajasthan)-333515, India

Received 6 December 2009; Accepted 2 February 2010

Copyright © 2010 Hindawi Publishing Corporation. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


A simple, precise, sensitive, fast and accurate high performance liquid chromatography method has been developed for the determination of atenolol using mixture of phosphate buffer and acetonitrile (53:47 v/v) as mobile phase. Buffer was prepared by mixing 0.02 M K2PO4 and 0.003 M KH2PO4 in equal proportion. Detection was carried out using UV detector at λmax 230 nm. Column was ODS and dimensions of column was 25 mm × 4.6 mm. Atenolol was eluted out at retention time of 2.1 min. Method was validated at 1.2 mL/min flow rate. Calibration curve was linear between ranges of 40 to 200 mcg concentration. The limit of detection was calculates 120 nano gram and limit of quantitation is 510 nano gram. The relative standard deviation (RSD) of atenolol was 0.6. The percentage recovery of atenolol was 99.6%.