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E-Journal of Chemistry
Volume 7 (2010), Issue 3, Pages 751-756

Liquid Chromatographic Method for Determination of Nisoldipine from Pharmaceutical Samples

Amit Gupta, Ram S. Gaud, and S. Ganga

School of Pharmacy and Technology Management, SVKM’s NMIMS University, V. L. Mehta Road, Vile Parle (W), Mumbai-56, India

Received 21 September 2009; Revised 25 November 2009; Accepted 10 January 2010

Copyright © 2010 Hindawi Publishing Corporation. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


A simple and specific high performance thin layer chromatographic method was developed and validated for the determination of nisoldipine from tablet dosage form. The method was carried out at 320 nm after extraction of drug in methanol. The method uses aluminum plates pre-coated with silica gel 60F-254 as stationary phase and cyclohexane-ethyl acetate-toluene (3:3:4, v/v/v) as mobile phase. Linearity was established over a range of 400-2400 ng per zone. Both peak area ratio and peak height ratio showed acceptable correlation coefficient i.e. more than 0.99. However we used peak area for validation purpose. Intra-day and inter-day precision was determined and found to have less than 6.0 % RSD.