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E-Journal of Chemistry
Volume 8, Issue 3, Pages 1451-1455
http://dx.doi.org/10.1155/2011/183172

In Vitro Antioxidant Capacity and Neuronal Cell Toxicity of Roots of Ostericum koreanum Maximowicz

Ramalingam Mahesh,1 Hyo Won Jung,2 Jun Hong Park,3 and Yong-Ki Park1,2

1Oriental Medicine Research Institute, Dongguk University, 707, Seokjangdong Gyeongju, Gyeongbuk 780-714, Republic of Korea
2Oriental Medicine R&D Center and Department of Herbology, College of Oriental Medicine, Dongguk University, 707, Seokjangdong Gyeongju, Gyeongbuk 780-714, Republic of Korea
3Bonghwa Alpine Medicinal Plant Experiment Station, Gyeongsangbook-do 755-843, Republic of Korea

Received 3 March 2010; Accepted 21 May 2010

Copyright © 2011 Hindawi Publishing Corporation. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Ostericum koreanum maximowicz (Umbelliferae), a medicinal herb in Korean Oriental Medicine, has been applied to treat cold, headache, neuralgia and arthralgia. The ethyl acetate fraction of O. koreanum root was subjected to in vitro antioxidant activity with different methods for free radical scavenging activities. In addition, the cell viability and nitric oxide release assays were performed here for the first time in neuroblastoma (Neuro-2a) cell cultures. Among all the tested methods, the ethyl acetate fraction was expressed very active, exhibiting a good Trolox equivalent values and IC50, comparable to that of the commercial antioxidants, Trolox and ascorbic acid, respectively. The results showed that there was a reduction of cell viability by the fraction in a concentration dependent manner. These results suggest that O. koreanum shows good antioxidant activities in vitro by inhibiting free radicals. These findings provide a rationale for the in vivo testing. Also, the major constituents behind the antioxidant mechanisms of this fraction warrant further study.