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E-Journal of Chemistry
Volume 8 (2011), S1, Pages S372-S376

Determination of Arctiin and Arctigenin Contents in Arctium Tomentosum Mill. by HPLC Method

Xiaoying Zhou,1,3 Haoke Zhang,1 Liang Ge,1 Haiyan Gong,1 and Shuge Tian1,2

1Xinjiang Key Laboratory of Famous Prescription and Science of Formulas, Urumqi-830011, Xinjiang, China
2College of TCM, Xinjiang Medical University, Urumqi-830011, Xinjiang, China
3College of Pharmacy, Xinjiang Medical University, Urumqi-830011, Xinjiang, China

Received 18 April 2011; Accepted 3 July 2011

Copyright © 2011 Hindawi Publishing Corporation. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


A simple, precise, rapid and accurate, binary-phase high performance liquid chromatographic method has been developed for the determination of arctiin and arctigenin contents in the Arctium tomentosumMill. with short run time. Chromatographic separation was achieved by using HPLC system, consisting of a Shimadzu LC-6AD and Kromasil C18 column (250×4.6 mm, 5 μm, with pre-column), the mobile phase consists of methanol and water (55: 45). Detection wavelength was 280 nm. The speed of flow was 1.0 mL/min. The specimen handing quantity was 10 μL. The arctiin’s linearity range was 1.575∼4.725 μg (r=0.9995). The arctigenin’s linearity range was 0.613, 3.063 μg (r = 0.9998) and the linear relationship was accurate. The average recovery (n=5) of arctiin and arctigenin were 101.55% (RSD=2.23%) 101.63% (RSD =1.49 %) respectively. The contents of arctiin and arctigenin in Arctium tomentosum Mill. were 10.69 mg/g and 0.15 mg/g, respectively. Therefore, the developed HPLC method can be applied to both in vitro studies of arctiin and arctigenin formulations as well as drug estimation in biological samples.