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E-Journal of Chemistry
Volume 8, Issue 4, Pages 1958-1964
http://dx.doi.org/10.1155/2011/784807

RP-HPLC Determination of Atomoxetine Hydrochloride in Bulk and Pharmaceutical Formulations

H. R. Prajapati, P. N. Raveshiya, and J. M. Prajapati

Department of Pharmaceutical Chemistry, A. R. College of Pharmacy, Vallabh Vidyanagar-388 120, Gujarat, India

Received 19 December 2010; Accepted 28 February 2011

Copyright © 2011 Hindawi Publishing Corporation. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

A reversed phase high performance liquid chromatographic (RP–HPLC) method was developed and subsequently validated for the determination of atomoxetine hydrochloride in bulk and pharmaceutical formulation. The separation was done by a PerkinElmer Brownlee analytical C8 column (260 mm x 4.6 mm, 5 µm) using methanol: 50 mM KH2PO2 buffer (PH adjusted to 6.8 with 0.1 M NaOH), 80:20 v/v as an eluent. UV detection was performed at 270 nm at a flow rate 1.0 mL/min. The validation of the method was performed, and specificity, reproducibility, precision accuracy and ruggedness were confirmed. The correlation coefficient was found to be 0.997 for atomoxetine hydrochloride. The recovery was in the range of 99.94 to 100.98% and limit of quantification was found to be 5.707 µg/mL. The method is simple, rapid, selective and economical too and can be used for the routine analysis of drug in pharmaceutical formulations.