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E-Journal of Chemistry
Volume 9, Issue 2, Pages 999-1006

Isocratic RP-HPLC Method for Simultaneous Separation and Estimation of Zofenopril and Hydrochlorthiazide in Pharmaceutical Dosage Forms

1Department of Pharmaceutical Chemistry, Malla Reddy College of Pharmacy, Maissamaguda, Dullapally, Secunderabad-14 Andrapradesh, India
2Department of Pharmaceutical Chemistry, Sultan Ul Uloom College of Pharmacy, Road No3, Banjara Hills, Secunderabad-500034 Andra Pradesh, India

Received 31 October 2011; Accepted 1 February 2012

Copyright © 2012 Hindawi Publishing Corporation. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


A simple, rapid, sensitive and accurate isocratic reverse phase high performance liquid chromatographic (RP-HPLC) method has been developed and subsequently validated for the simultaneous determination of Zofenopril and Hydrochlorthiazide in combined dosage form. Chromatographic separation of the two drugs was performed on a Purospher BDS C18 column (250 mm × 4.6 mm id, 5 μm particle size). The mobile phase comprising of acetonitrile methanol: 0.02M NaH22PO4 buffer (40:20:40) was delivered at a flow rate of 1.0mL/min. The pH of the mobile phase is adjusted to 7.2 with Sodium hydroxide solution. Detection was performed at 245 nm.The separation was completed within 10 min and the retention time of hydrochlorthiazide is 4.62 and Zofenopril is 6.86 min respectively. Calibration curves were linear with R2 between 0.99-1.0 over a concentration range of 100-600 μg/ml for Zofenopril calcium and 50-300 μg/ml for hydrochlorthiazide..The developed method was successfully applied to determi