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E-Journal of Chemistry
Volume 9 (2012), Issue 1, Pages 313-317
http://dx.doi.org/10.1155/2012/616431

High Performance Thin Layer Chromatographic Determination of Chrysin in Oroxylum Indicum Vent. from Different Geographical Regions of India

K. Srilatha Srinivas and A. Saraf Aparna

Department of Botany, The Institute of Science, Mumbai-32, India

Received 24 May 2011; Accepted 16 July 2011

Copyright © 2012 Hindawi Publishing Corporation. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

HPTLC fingerprints can be used for documentation and quantification of chemical markers to identify morphological and geographical variation in herbal raw materials. The current work was aimed at developing phytochemical fingerprints of Oroxylum indicum vent. from different geographical regions of India viz. Western Ghats (Maharashtra) and Northern Uttar Pradesh. Fingerprint of flavonoid, chrysin was developed for roots, leaves and bark of the plant from different regions using cold extraction and suitable solvent system. HPTLC technique was successfully used for evaluating regional variations. The fingerprints were also utilized for quantification of the flavonoid, chrysin from different parts of the plants from two distinct geographical regions. Maximum content of chrysin was found in plant collected from Western Ghats than in U.P.