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E-Journal of Chemistry
Volume 9 (2012), Issue 4, Pages 2052-2057

Improvement of Aspergillus niger for Sodium Gluconate Synthesis by UV Mutation Method

1Department of Bioprocess Technology, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia
2Department of Bioscience, Christian Eminent Academy, Indore 452001, India
3Institute of Bioscience, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia

Received 24 November 2011; Accepted 18 January 2012

Copyright © 2012 Hindawi Publishing Corporation. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


The sodium gluconate synthesis pathway was improved in Aspergillus niger by random mutation method. A. niger was mutated with Ultraviolet (UV) radiation and the alteration of cell bound enzymes activity of gluconic acid synthesis pathway and sodium gluconate synthesis were evaluated. The improved mutants (A. niger UV-112) was capable of producing sodium gluconate up to final concentrations of 60 g/L in batch fermentation, which was 3.0 fold higher than the parent strain. It have been observed that the changes in activities of cell bound enzymes related for gluconic acid pathway such as glucose dehydrogenase and glucose oxidase and it were significantly higher than the parent strain. The mutant A. niger strain and the simple method used to decrease the production cost and development of fermentation process for industrial production of gluconic acid or its salt.