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E-Journal of Chemistry
Volume 9 (2012), Issue 2, Pages 993-998
http://dx.doi.org/10.1155/2012/856130

Development and Validation of a Spectrophotometric Method for Quantification and Dissolution Studies of Glimepiride in Tablets

1Department of Pharmaceutical Chemistry, Malla Reddy College of Pharmacy, Maisammaguda, Dhulapally, Secunderabad 500014, India
2Department of Pharmaceutical Chemistry, C.M. College of Pharmacy, Maisammaguda, Dhulapally, Secunderabad 500014, India
3Department of Pharmaceutical Chemistry, A. U. College of Pharmaceutical Sciences, Andhra University, Visakhapatnam 530003, Andhra Pradesh, India

Received 10 July 2011; Accepted 20 September 2011

Copyright © 2012 Hindawi Publishing Corporation. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

The objective of present study was to develop and validate an analytical method for quantitative determination and dissolution studies of glimepiride in tablets. The glimepiride shows absorption maxima at 225 nm and obeyed Beer's law in the range of 6.0 – 14.0 µg/mL. The limit of detection and limit of quantitation were 0.06, and 0.17 µg/mL respectively. Percentage recovery of glimepiride for the proposed method ranged from 99.32 to 100.98% indicating no interference of the tablet excipients. It was concluded that the proposed method is simple, easy to apply, economical and used as an alternative to the existing spectrophotometric and non-spectrophotometric methods for the routine analysis of glimepiride in pharmaceutical formulations and in vitro dissolution studies.