Journal of Chemistry

Journal of Chemistry / 2012 / Article

Open Access

Volume 9 |Article ID 976242 | https://doi.org/10.1155/2012/976242

Harinath Dwivedi, Kusum Agrawal, Shubhini A Saraf, "Evaluation of Factors Affecting Uricase Production by the Screened Wild/Natural Microbes", Journal of Chemistry, vol. 9, Article ID 976242, 10 pages, 2012. https://doi.org/10.1155/2012/976242

Evaluation of Factors Affecting Uricase Production by the Screened Wild/Natural Microbes

Received15 Nov 2011
Accepted16 Jan 2012

Abstract

Gout is a metabolic disorder due to the deposition of uric acid crystals within articular or periarticular tissues. Uricase (urate oxidase) catalyzes the oxidation of less water soluble uric acid (7 mg/dl) to a compound allantoin which is more water soluble(11g/L at 40°C) resulting into the ease of excretion of uric acid. The objective of the work was to develop a new method for screening of microbes for uricase production and estimation of uricase thereof. This was achieved by utilizing the fact that uric acid dissolves on being acted upon by uricase. The proposed method is a novel, inexpensive, simple and sensitive technique for screening and estimation of uricase. Biomass and uricase production at different stages of microbial growth curve for the uricase producing microbe was studied. Effects of different medium components affecting uricase production by microbes were studied using Placket Burman statistical design. Addition of uric acid in the nutrient medium was found to be effective in increasing the uricase production by microbes growing in the medium.

Copyright © 2012 Hindawi Publishing Corporation. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


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