Validated Spectrophotometric Methods for Simultaneous Determination of Food Colorants and Sweeteners

Table 2

Method validation for the determination of laboratory prepared mixtures of IND, BB, ACE-K and ASP by the proposed methods.

Parameter	Accuracy mean* ± RSD%	Precision repeatability^a  ± RSD%	Intermediate precision^b  ± RSD%
ZCDS
IND (^1D348)	100.76±0.77	99.95±0.58	100.28±0.24
BB (^1D420)	99.96±0.73	100.68±0.58	99.86±0.31
ACE-K (^1D242)	100.65±0.49	100.05±0.09	100.09±0.17
ASP (^3D227)	101.61±1.76	101.05±0.50	100.57±0.81
RDS
IND (^1D356)	99.88±1.21	100.38±0.10	100.21±0.34
BB (^1D419)	98.91±0.48	99.78±0.26	100.67±0.83
ACE-K (^1D248)	98.92±0.48	100.13±0.32	99.95±0.85
ASP (^3D225)	98.54±1.60	100.45±0.40	100.39±0.53

^aThe intra-day (𝑛=5), average of three concentrations (2, 6, 10 µgmL⁻¹) for IND, BB, ACE-K and ASP repeated three times within the day.
^bThe inter-day (𝑛=5), average of three concentrations (2, 6, 10 µgmL⁻¹) for IND, BB, ACE-K and ASP repeated three times in three successive days.
*The values of % recovery is an average of five determinations of each of five concentration (2–10 µgmL⁻¹).
RSD%: Relative standard deviation.