In Vitro Antioxidant and Anticancer Activity of Flavonoid Fraction from the Aerial Parts of Cissus quadrangularis Linn. against Human Breast Carcinoma Cell Lines
Table 1
Isolation of total flavonoid fraction from the ethanol extract of Cissus quadrangularis.
S. no
Fraction
Eluates
TLC
value
Nature of the compound
1
1–5
Hexane (100)
No distinct spot
—
—
2
5–10
Hexane : chloroform (75 : 25)
No distinct spot
—
—
3
11–14
Hexane : chloroform (50 : 50)
No distinct spot
—
—
4
15–19
Hexane : chloroform (25 : 75)
No distinct spot
—
—
5
20–25
Chloroform (100)
Single spot
0.09
Negative for flavonoid test
6
26–32
Chloroform : ethyl acetate (75 : 25)
No distinct spot
—
—
7
33–40
Chloroform : ethyl acetate (50 : 50)
Single spot
0.11
Negative for flavonoid test
8
41–64
Chloroform : ethyl acetate (25 : 75)
Single spot
0.12
Positive for flavonoid test yellowish amorphous powder
9
65–70
Ethyl acetate (100%)
Single spot
0.43
Positive for flavonoid test yellowish brown amorphous powder
