Journal of Chemistry / 2013 / Article / Tab 1

Research Article

In Vitro Antioxidant and Anticancer Activity of Flavonoid Fraction from the Aerial Parts of Cissus quadrangularis Linn. against Human Breast Carcinoma Cell Lines

Table 1

Isolation of total flavonoid fraction from the ethanol extract of Cissus quadrangularis.

S. noFractionEluatesTLC valueNature of the compound

11–5Hexane (100)No distinct spot
25–10Hexane : chloroform
(75 : 25)
No distinct spot
311–14Hexane : chloroform
(50 : 50)
No distinct spot
415–19Hexane : chloroform
(25 : 75)
No distinct spot
520–25Chloroform (100)Single spot0.09Negative for flavonoid test
626–32Chloroform : ethyl acetate
(75 : 25)
No distinct spot
733–40Chloroform : ethyl acetate
(50 : 50)
Single spot0.11Negative for flavonoid test
841–64Chloroform : ethyl acetate
(25 : 75)
Single spot0.12Positive for flavonoid test yellowish amorphous powder
965–70Ethyl acetate (100%)Single spot0.43Positive for flavonoid test yellowish brown amorphous powder
1071–75Ethyl acetate : methanol
(75 : 25)
No distinct spot
1176–81Ethyl acetate : methanol
(50 : 50)
Single spot0.54Positive for flavonoid test brown sticky residue
1282–86Ethyl acetate : methanol
(25 : 75)
No distinct spotBrown sticky residue
1387–93Methanol (100%)Single spot0.66Positive for flavonoid test brown sticky residue

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