In Vitro Antioxidant and Anticancer Activity of Flavonoid Fraction from the Aerial Parts of Cissus quadrangularis Linn. against Human Breast Carcinoma Cell Lines
Table 2
Free radicals scavenging property of ethanolic extract and isolated flavonoid fraction of Cissus quadrangularis Linn.
Test
Concentration (µg/mL)
Nitric oxide inhibition (%)
DPPH radical inhibition (%)
Hydroxyl radical inhibition (%)
Ethanol extract
25
34.15 ± 1.26**
27.67 ± 1.17**
36.57 ± 0.54**
50
48.18 ± 0.36**
38.05 ± .25**
48.16 ± 1.40**
100
51.84 ± 2.72**
43.63 ± .58**
53.32 ± 1.08**
200
69.24 ± 1.96**
61.80 ± 2.43**
63.21 ± 1.72**
400
74.32 ± 2.16**
73.67 ± 0.35**
78.96 ± 0.61**
IC50
98 µg/mL
125 µg/mL
96 µg/mL
Flavonoid fraction
5
32.58 ± 0.58**
28.05 ± 1.20**
35.45 ± 1.20**
10
50.24 ± 3.28**
45.23 ± 2.18**
50.32 ± 1.50**
20
67.38 ± 0.54**
64.80 ± 1.43**
62.24 ± 3.28**
40
72.26 ± 0.86**
73.67 ± 2.85**
69.38 ± 0.54**
80
82.89 ± 0.97**
80.89 ± 1.40**
79.26 ± 0.86**
IC50
10 µg/mL
12 µg/mL
10 µg/mL
Standard quercetin
5
28.30 ± 1.08**
30.25 ± 0.47**
34.25 ± 0.47**
10
35.25 ± 0.47**
41.10 ± 2.16**
42.10 ± 3.16**
20
44.10 ± 2.16**
49.66 ± 1.12**
48.66 ± 1.12**
40
56.66 ± 1.12**
67.09 ± 0.16**
62.29 ± 0.54**
80
71.79 ± 0.54**
75.25 ± 2.47**
73.25 ± 0.47**
IC50
35 µg/mL
25 µg/mL
32 µg/mL
Values are mean ± SEM, statistical significant test for comparison was done by ANOVA, followed by Dunnet’s -test (). The values are when compared against standard.
