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Journal of Chemistry
Volume 2013, Article ID 184356, 5 pages
http://dx.doi.org/10.1155/2013/184356
Research Article

Reversed-Phase Liquid Chromatographic Separation and Determination of Ni(II), Cu(II), Pd(II), and Ag(I) Using 2-Pyrrolecarboxaldehyde-4-phenylsemicarbazone as a Complexing Reagent

1M. A. Kazi Institute of Chemistry, University of Sindh, Jamshoro 76080, Pakistan
2National Center of Excellence in Analytical Chemistry, University of Sindh, Jamshoro 76080, Pakistan
3Institute of Plant Sciences, University of Sindh, Jamshoro 76080, Pakistan

Received 12 June 2012; Accepted 3 October 2012

Academic Editor: Aleš Imramovsky

Copyright © 2013 Arfana Mallah et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

This paper reports the utilization of 2-pyrrolecarboxaldehyde-4-phenylsemicarbazone (PPS) as a complexing reagent for the simultaneous determination and separation of Ni(II), Cu(II), Pd(II), and Ag(I) by reversed-phase high-performance liquid chromatography with UV detector. A good separation was achieved using Microsorb C18 column (150 × 4.6 mm i.d.) with a mobile phase consisted of methanol : acetonitrile : water : sodium acetate (1 mM) (68 : 6.5 : 25 : 0.5 v/v/v/v) at a flow rate of 1 mL/min. The detection was performed at 280 nm. The linear calibration range was 2–10 μg/mL for all metal ions. The detection limits (S/N = 3) were 80 pg/mL for Ni(II), 0.8 ng/mL for Cu(II), 0.16 ng/mL for Pd(II), and 0.8 ng/mL for Ag(I). The applicability and the accuracy of the developed method were estimated by the analysis of Ni(II) in hydrogenated oil (ghee) samples and Pd(II) in palladium charcoal.