Reversed-Phase Liquid Chromatographic Separation and Determination of Ni(II), Cu(II), Pd(II), and Ag(I) Using 2-Pyrrolecarboxaldehyde-4-phenylsemicarbazone as a Complexing Reagent

Table 2

Quantitative HPLC data for metal ions Cu(II), Ni(II), Pd(II), and Ag(I) using PPS complexing reagent.

S. no.	Metal ion	Eluent	pH	Calibration range μg/mL	Detection limits
1	Ni(II)	M : W : A : Sod.Acet.*	8.0	2–10 𝜇g/mL	80 pg/mL
2	Cu(II)	M : W : A : Sod.Acet.*	8.0	2–10 𝜇g/mL	0.8 ng/mL
3	Pd(II)	M : W : A : Sod.Acet.*	5.0	2–10 𝜇g/mL	0.16 ng/mL
4	Ag(I)	M : W : A : Sod.Acet.*	6.0	2–10 𝜇g/mL	0.8 ng/mL

∗M: methanol, W: water, A: acetonitrile, Sod.Acet: sodium acetate.