Review Article

An Overview of Analytical Determination of Diltiazem, Cimetidine, Ranitidine, and Famotidine by UV Spectrophotometry and HPLC Technique

Table 3

Application of HPLC to the determination of H2 receptor antagonists.

ColumnMobile phaseDetectionRef.

250 × 4.6 mm I.D Nucleosil C18 (5 μm) packed by ChromexACN : phosphate buffer, pH 6.2 (25 : 75, v/v)228 nm [137]
Phenomenex (Torrance, CA) C18 (4.6 × 250 mm), Prodigy5_m ODS (3), 100ACN : heptanesulfonic acid 20 mM sodium acetate buffer (23 : 77)228 nm[146]
μ-Bondapak C18 column21 mM disodium hydrogen phosphate : triethylamine : ACN (1000 : 60 : 150, v/v), pH 3.5.320 nm[160]
Supelcosil LC-CN column (5 μm, 250 × 4.6 mm I.D.) 0.05 M potassium dihydrogen phosphate : ACN (88 : 12, v/v) pH 6.5229 nm[162]
Spherisorb phenyl cartridge column, 10 cm × 0.46 cm I.D., 5 μm particle diameter 88-89% of 0.02 M phosphate buffer pH 3 and 11-12% of methanol228 nm[167]
Cation-exchange column0.1 M sodium acetate buffer (pH5) : CAN : tetrahydrofuran (56.5 : 36 : 7.5, v/v)230 nm[172]
5-μm ODS silica ACN-7 mM triethylammonium ion in phosphoric acid, pH 3.0 (30 : 70 v/v).315 nm[173]
Nucleosil C18 columnAmmonium acetate solution (0.5 M), acetonitrile, and methanol254 nm[176]
Phenomenex (Torrance, CA, USA) Prodigy ODS (3) (5 mm particle size, 25 cm × 3.9 mm I.D.) reversed-phase HPLC columnACN and heptanesulfonic acid (2.5 gL−1) in 20 mM sodium acetate buffer (23 : 77).267 nm[191]
Supelcosil LC18 columnACN : 0.1 M dihydrogen phosphate buffer containing 0.2% triethylamine, (13 : 87 v/v), pH 3.0265 nm[206]
Octadecyl silane-bonded silica columnMethanol : 0.02 M sodium acetate (pH 4.5) (1 : 4)254 nm[209]
Porous graphitic carbon (PGC) columnACN : water containing 0.5% pentane sulphonic acid, (50 : 50 v/v)265 nm[210]