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Journal of Chemistry
Volume 2013 (2013), Article ID 234178, 5 pages
http://dx.doi.org/10.1155/2013/234178
Research Article

Ultrasensitive Nano-ELISA for Detecting Sulfadimethoxine in Chicken Tissue

1State Key Lab of Food Science and Technology, School of Food Science and Technology, Jiangnan University, Wuxi 214122, China
2Animal, Plant & Food Inspection Center, Jiangsu Entry-Exit Inspection and Quarantine Bureau, Nanjing 200001, China

Received 23 September 2013; Revised 3 December 2013; Accepted 8 December 2013

Academic Editor: Pranav S. Shrivastav

Copyright © 2013 Chi-Fang Peng et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

It is challenging to obtain a highly sensitive enzyme-linked sorbent immunoassay (ELISA) method for the rapid screening of veterinary drug residue in animal tissues. Here we present that a simple and sensitive detection for sulfadimethoxine (SDM) residue in animal tissues was achieved by utilizing a bioconjugate of gold nanoparticles and enzyme-labeled antibody as signal probe in enzyme-linked sorbent immunoassay (ELISA). The developed nano-ELISA has increased the sensitivity of a traditional ELISA for SDM by 20-fold. The sensitivity of this ELISA was 5 pg/mL in buffer, and the detection limit (LOD) of 0.2 µg/kg can be obtained after chicken liver was simply extracted by buffer. This simple and sensitive method can be used to improve the sensitivity of ELISA methods for various small molecule contaminants.