Stability-Indicating Validated HPLC Method for Analysis of Berberine Hydrochloride and Trimethoprim in Pharmaceutical Dosage Form

Table 2

Recovery data of berberine hydrochloride and trimethoprim from samples.

Compounds	Added amount; (𝜇g/mL)	Original amount; (𝜇g/mL)	Found amount; (𝜇g/mL)	Recovery; (%)	Mean recovery; (%)
	12	20	11.91	99.3
	12	20	11.97	99.7
	12	20	11.98	99.8
	20	20	19.74	98.7
Berberine hydrochloride	20	20	19.78	98.9	99.8
	20	20	19.77	98.8
	28	20	28.53	101.9
	28	20	27.88	99.6
	28	20	28.31	101.1
	6	10	6.07	101.2
	6	10	6.03	100.5
	6	10	6.11	101.8
	10	10	9.81	98.1
Trimethoprim	10	10	9.85	98.5	98.8
	10	10	9.75	97.5
	14	10	13.82	98.7
	14	10	13.46	96.1
	14	10	13.60	97.2