Poly-β-hydroxybutyrate (PHB) Depolymerase from Fusarium solani Thom

Table 2

Purification of PHB depolymerase of F. solani Thom.

Purification step	Enzyme activity; (U/mL)	Total enzyme activity; (U)	Protein; (mg/mL)	Total protein; (mg)	Specific activity; (U/mg)	Purification fold	Activity recovery; (%)
Crude enzyme (1000 mL)	5.0	5000	0.260	160	18.75	1.0	100
NH4SO4 ppt, dialysed (9.0 mL)	410	3690	2.5	28.8	128.125	6.83	73.8
Affinity chromatography (9 mL)	350	3150	0.115	1.035	3043.4	162.31	63