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Journal of Chemistry
Volume 2013 (2013), Article ID 451652, 9 pages
http://dx.doi.org/10.1155/2013/451652
Research Article

Modeling, Simulation, and Kinetic Studies of Solvent-Free Biosynthesis of Benzyl Acetate

Microbial Biotechnology and Downstream Processing Laboratory, Agricultural and Food Engineering Department, Indian Institute of Technology, Kharagpur, West Bengal 721302, India

Received 15 May 2013; Accepted 28 August 2013

Academic Editor: Jose M. Cruz

Copyright © 2013 Vijay Kumar Garlapati et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Solvent-free biosynthesis of benzyl acetate through immobilized lipase-mediated transesterification has been modeled and optimized through statistical integrated artificial intelligence approach. A nonlinear response surface model has been successfully developed based on central composite design with transesterification variables, namely, molarity of alcohol, reaction time, temperature, and immobilized lipase amount as input variables and molar conversion (%) as an output variable. Statistical integrated genetic algorithm optimization approach results in an optimized molar conversion of 96.32% with the predicted transesterification variables of 0.47 M alcohol molarity in a reaction time of 13.1 h, at 37.5°C using 13.31 U of immobilized lipase. Immobilized lipase withstands more than 98% relative activity up to 6 recycles and maintains 50% relative activity until 12 recycles. The kinetic constants of benzyl acetate, namely, and were found to be 310 mM and 0.10 mmol h−1 g−1, respectively.