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Journal of Chemistry
Volume 2013, Article ID 452610, 6 pages
Research Article

HPLC Quantitative Analysis of Main Stilbenes and Flavones in Different Parts of Matteuccia struthiopteris

1Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China
2Institute of Experimental Medicine, Academy of Sciences of the Czech Republic, Vídeòská 1083, 142 20 Prague, Czech Republic

Received 5 June 2013; Revised 29 September 2013; Accepted 11 October 2013

Academic Editor: Alexander Kornienko

Copyright © 2013 Shubei Li et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Supplementary Material

Accurately weighed six compounds were dissolved in methanol to prepare stock solutions, respectively. A certain amount of each stock solution was placed in a 25 mL volumetric flask and diluted to volume with methanol at the concentration of 433.20 μg/mL DMN, 409.20 μg/mL MN, 20.32 μ/mL MR, 42.72 μ/mL PV, 20.16 μ/mL PVG, and 71.60 μg/mL SCG. The mixture solution was then diluted stepwise with methanol to give six different concentrations for construction of calibration curves.

The standard mixture solutions at six different concentrations were injected in triplicate. The calibration curve was constructed by plotting six points of peak area (yaxis) versus concentration (xaxis) for each compound.

  1. Supplementary Materials