Research Article
Extractive Spectrophotometric Method for the Determination of Lamivudine and Zidovudine in Pharmaceutical Preparations Using Bromocresol Purple
Table 1
Optical characteristics and statistical data of the regression equations for ZVD and LVD with BCP.
| Parameter | LVD | ZVD |
| (nm) | 414 | 414 | Beer’s law limit (μg mL−1)b | 25–250 | 50–300 | Molar absorptivity (l mol−1 cm−1) | 3.68 × 103 | 3.047 × 103 | Sandell’s sensitivity (μg cm−2 per 0.001 absorbance unit) | 1.604 × 10−5 | 1.140 × 10−5 | Regression equationa | | | Slope ± SD | 0.0037 ± 0.00013 | 0.0036 ± 0.00026 | Intercept ± SD | −0.0153 ± 0.000054 | 0.0772 ± 0.000050 | Correlation coefficient, | 0.9995 | 0.9997 | LOD (μg mL−1) | 0.106 | 0.217 | LOQ (μg mL−1) | 0.352 | 0.722 | Intra-dayb | 0.65 | 0.54 | Inter-dayc | 0.87 | 0.71 |
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(where is the concentration of drug in g mL−1).
bAverage of six determinations.
cResults of seven different days.
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