Validated Spectrophotometric Methods for the Determination of Mycophenolate: An Anti-Neoplastic Agent in Bulk and Pharmaceutical Dosage Forms

Table 1

Optical characteristics and validation parameters of Mycophenolate.

Parameters	Method A	Method B	Method C
Beer-Lambert’s range (μg/mL)	1–150	1–150	1–150
𝜆max/wavelength range (nm)	250	258	240–260
Molar absorptivity (L/mol·cm)	9.71×10^3	7.37×10^2	4.68×10^4
Sandell’s sensitivity (μg cm^2/0.001 absorbance unit)	0.05	—	—
Slope	0.022	0.001	0.107
Standard deviation of slope	0.0252	0.0002	0.0036
Intercept	0.001	0.000	0.025
Standard deviation of intercept	0.0003	0.000	0.0025
Correlation coefficient	0.999	0.999	0.999
Precision (RSD, %)
Intraday (𝑛=3)	0.61–1.25	0.78–0.90	0.92–1.11
Interday (𝑛=3)	0.96–1.09	0.66–1.12	0.87–1.04
Accuracy (% recovery) (% RSD)	99.87–101.80 (0.23)	98.56–100.54 (0.41)	99.75–100.90 (0.38)