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Journal of Chemistry
Volume 2013 (2013), Article ID 561905, 7 pages
http://dx.doi.org/10.1155/2013/561905
Research Article

The Inhibitory Effects of Aqueous Extract from Guava Twigs, Psidium guajava L., on Mutation and Oxidative Damage

1Department of Health and Nutrition, Chia-Nan University of Pharmacy and Science, No. 60 Erh-Jen Rd., Sec.1, Tainan 717, Taiwan
2Department of Applied Life Science and Health, Chia-Nan University of Pharmacy and Science, 60 Erh-Jen Road, Section 1, Tainan 717, Taiwan
3Department of Food Science and Technology, Chia-Nan University of Pharmacy and Science, 60 Erh-Jen Road, Section 1, Tainan 717, Taiwan
4Department of Food Nutrition, Chung Hwa University of Medical Technology, 89 Wenhwa 1st Street, Tainan 717, Taiwan

Received 29 June 2012; Accepted 5 November 2012

Academic Editor: Mehmet Emin Duru

Copyright © 2013 Zhi-Chyang Kang et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

This study examines the inhibitory effects of the aqueous extract from guava twigs (GTE), Psidium guajava L., on mutation and oxidative damage. The results show that GTE inhibits the mutagenicity of 4-nitroquinoline-N-oxide (4-NQO), a direct mutagen, and 2-aminoanthracene (2-AA), an indirect mutagen, toward Salmonella typhimurium TA 98 and TA 100. In addition, GTE shows radical scavenging, reducing activities, tyrosinase inhibition, and liposome protection effects. Meanwhile, GTE in the range of 0.1–0.4 mg/mL protects liver cells from tert-butyl-hydroperoxide-(t-BHP-) induced cytotoxicity. Furthermore, the cytotoxicity inhibition of GTE in the t-BHP-treated cells was demonstrated in a dose-dependent manner. High-performance liquid chromatography analysis suggests that the major phenolic constituents in GTE are gallic acid, ferulic acid, and myricetin. These active phenolic components may contribute to the biological protective effects of GTE in different models. The data suggest that GTE exhibiting biological activities can be applied to antimutation, antityrosinase, and antioxidative damage.