Table 9: Effect of various parameters in assessment of method.

ParametersVariationObserved values
R.TT.PTailingResolution

Flow rate0.5 mL/min3.6359421.2210.86
0.7 mL/min3.6259101.1910.23
Column temperature20°C3.6263081.2511.22
30°C3.7561151.229.79
Mobile phase90% organic3.6359601.2310.79
110% organic3.6258401.259.29