Development and Validation of a Method for the Quantification of an Alkaloid Fraction of Himatanthus lancifolius (Muell. Arg.) Woodson by Ultraviolet Spectroscopy

Table 2

Data of the robustness of the developed UV spectrophotometric method for the determination of total alkaloids in the aqueous extract of H. lancifolius.

Calculated concentration (𝜇g/mL)	Solvent	𝑁	𝐶 (𝜇g/mL)	Absorbance
16	1	3	16.448	0.293
20		3	20.580	0.373
28		3	28.373	0.523
16	2	3	16.217	0.261
20		3	19.651	0.315
28		3	28.141	0.449

𝐶 = average concentration of 𝑁 determinations.