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Journal of Chemistry
Volume 2013, Article ID 784543, 4 pages
http://dx.doi.org/10.1155/2013/784543
Research Article

Analysis of the Kanamycin in Raw Milk Using the Suspension Array

1Institute of Instrument Technology, Chinese Academy of Inspection and Quarantine, Beijing 100123, China
2General Administration of Quality Supervision Inspection and Quarantine of the People’s Republic of China, Beijing 100088, China

Received 20 June 2012; Accepted 2 August 2012

Academic Editor: Dimosthenis L. Giokas

Copyright © 2013 Yanfei Wang et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

With the monoclonal antibody against kanamycin being prepared successfully, a bead-based indirect competitive fluorescent immunoassay was developed to detect kanamycin in milk. The fact that there was no significant cross-reaction with other aminoglycoside antibiotics implied that the monoclonal antibody was highly specific for kanamycin. The limit of detection (LOD) and the 50% inhibition concentration (IC50) in raw milk were 3.2 ng/mL and 52.5 ng/mL, respectively. Using the method developed in this study, the kanamycin concentrations were monitored in raw milk after the intramuscular administration of kanamycin in sick cows. Compared to the conventional enzyme-linked immunosorbent assay (ELISA), the method using the suspension array system was more sensitive. The results obtained in the present study showed a good correlation with that of the ELISA.