Research Article
Analysis of the Kanamycin in Raw Milk Using the Suspension Array
Table 2
Intra- and inter-assay validation of the microsphere-based fluorescence immunoassay.
| | Parameter | Kanamycin concentration (ng/mL) | | 1 | 2.5 | 5 | 10 | 25 | 50 | 100 | 1000 |
| | MFI/MFI0% (intra-assay) | 96.9 | 91.5 | 86.1 | 78.6 | 64.5 | 51.8 | 38.4 | 11.9 | | SD () | 2.8 | 7.9 | 5.3 | 3.0 | 0.7 | 3.4 | 4.6 | 2.5 | | Intra-CV% | 2.9 | 8.7 | 6.2 | 3.8 | 1.1 | 6.6 | 12.0 | 20.6 | | MFI/MFI0% (inter-assay) | 96.3 | 92.3 | 86.9 | 76.8 | 62.0 | 52.4 | 37.8 | 12.2 | | SD () | 1.8 | 2.4 | 7.9 | 3.5 | 2.4 | 5.1 | 4.3 | 2.0 | | Inter-CV% | 1.9 | 2.6 | 9.1 | 4.5 | 3.8 | 9.8 | 11.5 | 16.7 |
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