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Journal of Chemistry
Volume 2013, Article ID 825290, 8 pages
http://dx.doi.org/10.1155/2013/825290
Research Article

Antihyperlipidemic Potential of Polyphenol and Glycoside Rich Nerium oleander Flower against Triton WR-1339-Induced Hyperlipidemia in Experimental Sprague Dawley Rats

1Department of Biotechnology, Mother Terasa Women’s University, Kodaikanal 624102, India
2Department of Biotechnology, School of Life Sciences, Pondicherry University, Puducherry 605014, India

Received 11 February 2012; Revised 2 May 2012; Accepted 31 May 2012

Academic Editor: Devanand Luthria

Copyright © 2012 V. Gayathri et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Nerium oleander Linn. (NO), an evergreen cardiac glycoside-rich shrub is used as folklore medicine in China to treat many diseases. It exhibits a wide spectrum of bioactivities but there were not much scientific reports on the bioactivity of N. oleander flowers. In the present study, we have evaluated the toxicity profile of the 50% hydroethanolic extracts of Nerium oleander flowers (ENO) using in vitro brine shrimp lethality assay and MTT cytotoxicity assay and in vivo acute toxicity test as per the OECD guidelines. The antihyperlipidemic activity of the ENO was also studied using Triton WR-1339-induced hyperlipemic rats and compared with standard Atorvastatin. In vitro brine shrimp, MTT cytotoxic assay, and in vivo acute toxicity assays showed a wide safety margin which has been evidenced through its lethal concentration (LC50: 795.46 μg/mL) and growth inhibition (GI50: 993.60 μg/ml) values. Plasma lipids and lipoproteins were significantly elevated by the intraperitoneal injection of Triton WR 1339 in hyperlipidemic rats at 6th and 24th hour. ENO pretreatment showed a significant ameliorative action on elevated lipids and lipoproteins in a dose-dependent manner when compared to standard. Altogether, the results prove that Nerium oleander flowers are not toxic at the tested doses and exhibit antilipimic activity.