A Novel and Sensitive Method for the Determination of Vitamin B_𝟐 (Riboflavin) in Urine and Pharmaceutical Samples Using an Aqueous Two-Phase Extraction

Table 2

Determination of vitamin B₂ in urine and some pharmaceutical formulations by the proposed aqueous two-phase extraction (𝑛=3).

Sample	Added (ng/mL)	Found ± SD^a (ng/mL)	RSD %	Recovery %
	0	41.0±2.4	5.8	—
Ampoule	60	102.3±2.8	2.7	102.1
	100	145.0±3.1	2.1	103.98
	0	4.9±0.2	4.1	—
Capsule	60	66.3±2.8	4.2	102.28
	100	103.7±3.7	3.5	98.77
	0	146.1±3.3	2.2	—
Urine	60	208.3±3.2	1.5	103.66
	100	241.6±3.6	1.5	95.50
	0	0.00	—	—
Serum	60	58.1±2.8	4.8	96.83
	100	102.1±3.6	3.5	102.14
	0	0.00	—	—
Sandrous syrup	60	56.9±3.2	5.6	94.82
	100	96.3±4.1	4.2	96.33

aStandard deviation.