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Journal of Chemistry
Volume 2013, Article ID 938154, 6 pages
Research Article

Partitioning Behavior of Papain in Ionic Liquids-Based Aqueous Two-Phase Systems

1Key Laboratory of Tropical Medicinal Plant Chemistry of Education, Hainan Normal University, Haikou 571158, China
2College of Chemistry and Chemical Engineering, Jiangsu University, Xuefu Road 301, Zhenjiang 212013, China

Received 30 May 2013; Revised 15 August 2013; Accepted 18 August 2013

Academic Editor: M. Natália D. S. Cordeiro

Copyright © 2013 Zhiwen Bai et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


This paper attempts to study and optimize the affinity partitioning conditions of papain in an aqueous two-phase system (ATPS). The effect of the amount of ionic liquids (ILs), the concentration of K2HPO4, temperature, pH, and the volume of papain solution were discussed concretely. The optimum conditions were determined as ionic liquid was 1.4 g and K2HPO4 was 1.4 g, the extraction efficiency of papain could reach 98.33% with pH unadjusted. The temperature and the pH of the solution are major parameters that influence the partitioning of protein in ILs-based ATPSs. The partition of papain to the IL-rich phase was enhanced by increasing the amount of ILs, the concentration of K2HPO4, and temperature, especially at its isoelectric point.