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Journal of Chemistry
Volume 2013, Article ID 939215, 5 pages
http://dx.doi.org/10.1155/2013/939215
Research Article

Nematocidal Constituents from the Ethanol Extract of Evodia rutaecarpa Hort Unripe Fruits

Department of Entomology, China Agricultural University, 2 Yuanmingyuan West Road, Haidian District, Beijing 100193, China

Received 5 January 2012; Accepted 20 April 2012

Academic Editor: Javier Hernandez-Borges

Copyright © 2013 Qi Zhi Liu et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

The ethanol extract of Chinese medicinal herb, Evodia rutaecarpa Hort unripe fruits, was found to possess nematocidal activity against the root-knot nematodes, Meloidogyne incognita, during the screening program for new agrochemicals from local wild plants and Chinese medicinal herbs. Bioactivity-guided chromatographic separation of the ethanol extract of E. rutaecarpa on repeated silica gel columns led to isolate five constituent components (two limonoids, evodol and limonin; three alkaloids, evodiamine, rutaecarpine, and wuchuyuamide I). Evodiamine ( L C 5 0 = 7 3 . 5 5 μg/mL) and rutaecarpine ( L C 5 0 = 1 2 0 . 8 5 μg/mL) exhibited stronger nematocidal activity against M. incognita than the crude ethanol extract of E. rutaecarpa ( L C 5 0 = 1 3 1 . 5 4 μg/mL). Wuchuyuamide I, evodol, and limonin also possessed nematocidal activity against M. incognita with LC50 values of 147.87 μg/mL, 155.02 μg/mL, and 197.37 μg/mL, respectively, but weaker than the crude ethanol extract of E. rutaecarpa.